Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1, 1987 - 1988

Display Title

Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1, 1987 - 1988

Source

box: 05 folder: 05 (text)

Description

Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map
Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot tests. Documents of note is a correspondence from Garnet at Berkley to Greider, describing his cell colony, treated it through denaturing, drying, and neutralizing the colony.

Scope and Contents

Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map
Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot tests. Documents of note is a correspondence from Garnet at Berkley to Greider, describing his cell colony, treated it through denaturing, drying, and neutralizing the colony.

Preferred Citation

Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1, 1987 - 1988. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525506. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Category

Lab Notebooks

Correspondence

Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Component Unique Identifier

CWG_01_021

Reference ID

f78d85868a46c026b9b6b7c99f372c0d

Finding Aid Link

Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1