Course Notebook: Methods II Proteins, 1979 - 1988

Display Title

Course Notebook: Methods II Proteins, 1979 - 1988

Source

box: 09 folder: 03-04 (text)

Extent

2 Folders

Description

This is a notebook within a series on the methods that Greider used in her experiments. The notebook begins with protocols of Bio-Rad Protein Assay. The assay details components and reagents as well as different microassay tests. The notebook then moves on to another series of protocols. The first part of the protocol is for a mouse monoclonal IgG1 purification with Affi-Gel Protien A. The protocol provided improvement to the agarose gels that were used for gel electrophoresis. It also included Greider's notes on a series of chemical compatibility tests as well as electrophoresis calibration tests. The next series of documents were instructions on how to construct columns for liquid chromatography and for a mini-gel electrophoresis kit. The kit listed properties of standard protiens that could enter the gel alongside references of previous users of the kit. The notebook also contained a document for a protocal to prep cells that have been spheroplasted and lyticased, which essentially means it is a protocol to maintain the chromatin inside of the cell. An interesting historical note, the notebook also contains a letter. The letter is from a colleague of Greider's, Christopher Szent-Gyorgyi, who was a researcher at the University of Texas Health and Science Center at Dallas at the time, to Elizabeth Blackburn. The letter detailed a list of write ups to isolate yeast nuclei.

The next file in the same series begins by testing different levels of Acrylamide gels. Greider had an idea for using alternative technologies, other types of mini gels and and other gels besides the standard agarose. Also included instructions for prepping buffer solutions
with photo copied images of a book. The book was entitled Protein Assay by Dye Binding and it instruced the user on different methods of dye binding. Using these protocals Greider came up with two methods for extracting yeast DNA. One used spheroplasts and the other used glass beads. Greider also prepared a basic centrifuge that would spin the extracted solution and obtain the nucleate. Greider also spent some time describing the different types of rotors and adapters. The notebook concluded with instructions to separate proteins from nucleic acids and the types of reagents to use.

Scope and Contents

This is a notebook within a series on the methods that Greider used in her experiments. The notebook begins with protocols of Bio-Rad Protein Assay. The assay details components and reagents as well as different microassay tests. The notebook then moves on to another series of protocols. The first part of the protocol is for a mouse monoclonal IgG1 purification with Affi-Gel Protien A. The protocol provided improvement to the agarose gels that were used for gel electrophoresis. It also included Greider's notes on a series of chemical compatibility tests as well as electrophoresis calibration tests. The next series of documents were instructions on how to construct columns for liquid chromatography and for a mini-gel electrophoresis kit. The kit listed properties of standard protiens that could enter the gel alongside references of previous users of the kit. The notebook also contained a document for a protocal to prep cells that have been spheroplasted and lyticased, which essentially means it is a protocol to maintain the chromatin inside of the cell. An interesting historical note, the notebook also contains a letter. The letter is from a colleague of Greider's, Christopher Szent-Gyorgyi, who was a researcher at the University of Texas Health and Science Center at Dallas at the time, to Elizabeth Blackburn. The letter detailed a list of write ups to isolate yeast nuclei.

The next file in the same series begins by testing different levels of Acrylamide gels. Greider had an idea for using alternative technologies, other types of mini gels and and other gels besides the standard agarose. Also included instructions for prepping buffer solutions
with photo copied images of a book. The book was entitled Protein Assay by Dye Binding and it instruced the user on different methods of dye binding. Using these protocals Greider came up with two methods for extracting yeast DNA. One used spheroplasts and the other used glass beads. Greider also prepared a basic centrifuge that would spin the extracted solution and obtain the nucleate. Greider also spent some time describing the different types of rotors and adapters. The notebook concluded with instructions to separate proteins from nucleic acids and the types of reagents to use.

Preferred Citation

Course Notebook: Methods II Proteins, 1979 - 1988. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525524. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Reference ID

d038ab9d9d3fce43bfd95c373110eb69

Finding Aid Link

Course Notebook: Methods II Proteins